Highly flexible low volume agitating incubation station

Optimizing Fluorescence In-Situ Hybridization and Immunohistochemistry Tissue Incubation

The hybridization station SlideBooster™ SB450 improves biological results of open well experiments as well as all coverslip and lifterslip incubations. With two independent incubation chambers a total number of four slides can be hybridized in parallel. Microagitation chips integrated into the chamber floor generate the surface acoustic wave (SAW) that penetrates the slides and cause ‘quasi-chaotic’ streaming in the sample solution. This results in homogenous mixing. Due to the SAW technology, the hybridization is no longer diffusion limited resulting in shorter incubation times or higher quality in the same incubation time as present methods, using volumes as low as 5 µl.

The SlideBooster provides:

• Compatibility with all coverslip, geneframe and lifterslip experiments
• Hybridizations of tissue samples in an open well (Immunohistochemistry, FISH)
• Multiple Hybridization on a single slide
• Increased sensitivity
• Improved reproducibility
• Enhanced specifity

Other major advantages:

• No changes in protocols
• Simple Operation
• Significantly reduced assay time for Fluorescence In-situ Hybridizations (FISH) and Immunohistochemistry experiments
• No dead volume, minimal experiment volumes (less than 5 µl)
• Minimal cleaning or maintenance efforts
• Small Footprint

The SlideBooster™ is designed for the following applications:
• Fluorescence in situ hybridization (FISH) • Multi-color FISH • Immuno-histochemistry (IHC) • Comparative genomic hybridization on Chromosomes (CGH) • Chromosome staining